Human DCC-Interacting Protein 13-alpha (APPL1) ELISA Kit from MyBioSource.com

Supplier Page

Supplier Page from
MyBioSource.com for
Human DCC-Interacting Protein 13-alpha (APPL1) ELISA Kit

Get Pricing

Description

This assay employs a two-site sandwich ELISA to quantitate APPL1 in samples. An antibody specific for APPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured